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Karyotyping and Molecular Sexing in Brycinus nurse (Characiformes, Alestidae) from Nile River in Egypt.

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Alternate Title:
      انت صٌيف انكر مً سٌ مٌي اًنفر قً انجزيئيو بين انجنسين في سمك انراي من نير اننيم في مصر انسيد عبد انمنصف محمد ، نجلاء نجيب ظيري ، جمبل إبراىيم أحمد رأفت فؤاد عبده
    • الموضوع:
      EGYPT
    • نبذة مختصرة :
      The present study aimed to investigate the karyotype and molecular sex differences in Brycinus nurse. Analysis of 430 metaphases spreads from 12 specimens revealed that the diploid chromosome number is 44 chromosomes and the proposed karyotype formula is n = 3 M + 3 SM + 16 A, FN = 56. No morphologically differentiated heteromorphic sex chromosomes were observed. On the other hand, 29 primers of three molecular markers (RAPD, SCoT and ISSR) were tested to differentiate between males and females of B. nurse. From all tested primers only two primers (OPI-18 and SCoT-18) successfully generated female specific bands in the bulked DNA samples which further confirmed using individual DNA samples. According to molecular analysis result in this study it seems that females of B. nurse are the heterogametic gender (ZW) and males of B. nurse are the homogametic gender (ZZ). In the future, SCoT marker can be used as suitable and powerful marker in genetic analysis studies in fish. [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      اىتحييو اىنز سٍٗ٘ يٍ٘ . )Brycinus nurse( ت ذٖف اىذراس اىحبىي اىي اىتعزف عيي اىت ص٘يف اىنز سٍٗ٘ يٍ٘ اٗلإختلافبت اىجزيئي بي اىج سْي في س لَ اىزاي ىٗ يلاحع جٗ د٘ أي )n = 3 M + 3 SM + 16 A, FN = ى 731 يٍتبفيز 13 فزد أ اىعذد اىنز سٍٗ٘ يٍ٘ اىث بْئي 73 مز سٍٗ٘ أٗ اى يٖئ اىنز سٍٗ٘ يٍ٘ يٕ: ) 56 اىعشز SCoT مٗذىل أحذ ببدئبت اى )OPI- اىتسع اى سَتخذ ) 18 RAPD مز سٍٗ٘ بٍ٘ت جسْ يٍَز رٍ٘ف ى٘ ج٘يب 4 عٗيي اىجب بّ الآخز أظ زٖ مو :ٍِ أحذ ببدئبت اى اىعشز اى سَتخذ في إظ بٖر أي حز تٍخصص ىيجسْ 4 طٗبقب ى تْبئج اىتحييو ISSR حز اٗحذ تٍخصص ىلإ بّث 4 ىٗ ي جْح أي ببدئبت اٗس اى )SCoT- اى سَتخذ ) 18 إٗس ق ي٘ فٗعبه SCoT 4 اٗس اى )ZZ( أٗ اىذم ر٘ يٕ اىجسْ تٍ بَثو اىجب يٍطبت (ZW) اىجزيئي ى ذٖ اىذراس فإ الإ بّث في س لَ اىزاي يٕ اىجسْ خٍتيف اىجب يٍطبت يٗ نَ أ يستخذ سٍتقبلا في دراسبت اىتحييو اى ر٘اثي في الأس بَك 4 [ABSTRACT FROM AUTHOR]
    • نبذة مختصرة :
      Copyright of Journal of Agricultural Chemistry & Biotechnology is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)