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Bio-emulsifying and biodegradation activities of syringafactin producing Pseudomonas spp. strains isolated from oil contaminated soils.
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- المؤلفون: Zouari, Oumaima1,2 (AUTHOR); Lecouturier, Didier1,3 (AUTHOR) ; Rochex, Alice1 (AUTHOR); Chataigne, Gabrielle1 (AUTHOR); Dhulster, Pascal1 (AUTHOR); Jacques, Philippe1,4 (AUTHOR); Ghribi, Dhouha2 (AUTHOR)
- المصدر:
Biodegradation. Aug2019, Vol. 30 Issue 4, p259-272. 14p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Pseudomonas strains isolated from oil contaminated soils were screened for biosurfactant production. Three out of eleven Pseudomonas isolates were selected for their high emulsifying activity (E24 value on n-hexadecane ~ 78%). These isolates (E39, E311 and E313) were identified as members of the P. putida group using phenotypical methods and a molecular approach. To identify the chemical nature of produced biosurfactants, thin layer chromatography and MALDI-ToF mass spectrometry analysis were carried out and revealed lipopeptides belonging to the syringafactin family. The activity of the produced biosurfactants was stable over a pH range of 6–12, at high salinity (10%) and after heating at 80 °C. Tests in contaminated sand micro-bioreactors showed that the three strains were able to degrade diesel. These results suggest the potential of these syringafactin producing strains for application in hydrocarbon bioremediation. [ABSTRACT FROM AUTHOR]
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