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Saikosaponin-d inhibits proliferation by up-regulating autophagy via the CaMKKβ-AMPK-mTOR pathway in ADPKD cells.
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- المؤلفون: Shi, Weiwei1; Xu, Dechao1; Gu, Junhui1; Xue, Cheng1; Yang, Bo1; Fu, Lili1; Song, Shuwei1; Liu, Dongmei1; Zhou, Wei1; Lv, Jiayi1; Sun, Ke1; Chen, Meihan1; Mei, Changlin1
- المصدر:
Molecular & Cellular Biochemistry. Dec2018, Vol. 449 Issue 1/2, p219-226. 8p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Autosomal dominant polycystic kidney disease (ADPKD) is a common heritable human disease. Recently, the role of repressed autophagy in ADPKD has drawn increasing attention. Here, we investigate the mechanism underlying the effect of Saikosaponin-d (SSd), a sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump (SERCA) inhibitor. We show that SSd suppresses proliferation in ADPKD cells by up-regulating autophagy. We found that treatment with SSd results in the accumulation of intracellular calcium, which in turn activates the CaMKKβ-AMPK signalling cascade, inhibits mTOR signalling and induces autophagy. Conversely, we also found that treatment with an autophagy inhibitor (3-methyladenine), AMPK inhibitor (Compound C), CaMKKβ inhibitor (STO-609) and intracellular calcium chelator (BAPTA/AM) could reduce autophagy puncta formation mediated by SSd. Our results demonstrated that SSd induces autophagy through the CaMKKβ-AMPK-mTOR signalling pathway in ADPKD cells, indicating that SSd might be a potential therapy for ADPKD and that SERCA might be a new target for ADPKD treatment. [ABSTRACT FROM AUTHOR]
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