Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print Cited Medium: Print ISSN: 0021-924X (Print) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE

Publication: : Abingdon, UK : Oxford University Press
Original Publication: Tokyo : Japanese Biochemical Society

Mitochondrial ADP, ATP Translocases/*biosynthesis ; Mitochondrial ADP, ATP Translocases/*genetics ; Testis/*enzymology; Alternative Splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological Transport ; Exons/genetics ; Female ; Genes/genetics ; Germ Cells/metabolism ; Isoenzymes/biosynthesis ; Isoenzymes/genetics ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism ; Molecular Sequence Data ; Ornithine Carbamoyltransferase/metabolism ; RNA, Messenger/biosynthesis ; Testis/physiology

The 34-kDa translocase of the outer mitochondrial membrane (Tom34) is a putative mammalian-specific factor involved in protein import into mitochondria. We analyzed the genomic sequence of the mouse Tom34 gene and found it has two alternative initial exons. Using reverse transcription and the polymerase chain reaction (RT-PCR), we found that these two mRNAs differs only in the 5'-proximal sequences corresponding to the two initial exons (exon 1a and 1b). Tom34 mRNA with exon 1a (Tom34a) is expressed ubiquitously, while that with exon 1b (Tom34b) is expressed only in mature testicular germ cells. To explore the in vivo function of Tom34 proteins, we generated Tom34-deficient mice by targeted disruption. The Tom34(-/-) mice were viable and grew normally and had a normal Mendelian inheritance pattern. Male as well as female Tom34(-/-) mice were fertile. In vitro-preprotein import into isolated mitochondria showed no apparent difference between Tom34(-/-) and wild-type mice. These results indicate that Tom34 is dispensable for mouse growth and development under optimal conditions.

0 (Isoenzymes)
0 (RNA, Messenger)
9068-80-8 (Mitochondrial ADP, ATP Translocases)
EC 2.1.3.3 (Ornithine Carbamoyltransferase)

Date Created: 20030613 Date Completed: 20040413 Latest Revision: 20190513

20231215

10.1093/jb/mvg080

12801914