Identification and characterization of a shuttle plasmid with antibiotic resistance gene from Staphylococcus aureus.

Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Print Cited Medium: Print ISSN: 0343-8651 (Print) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE

Original Publication: New York, Springer International.

Drug Resistance, Multiple, Bacterial/*genetics ; R Factors/*genetics ; Staphylococcus aureus/*genetics; Amino Acid Sequence ; Base Sequence ; Blotting, Southern ; Chloramphenicol O-Acetyltransferase/genetics ; Genetic Vectors/genetics ; Humans ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Replication Origin/genetics ; Sequence Homology, Nucleic Acid ; Staphylococcus aureus/metabolism ; Transformation, Bacterial/genetics

While studying antibiotic-resistant plasmids from multi-drug-resistant nosocomial Staphylococcus aureus strains, we isolated a small (2.889 kb) chloramphenicol-resistant (Cm(r)) plasmid, which was designated as pMC524/MBM. The molecular size of pMC524/MBM was close to that of pC194 (2.910 kb), a well-known Cm(r) staphylococcal plasmid. Unlike pC194, this plasmid can replicate and express itself efficiently and stably in Escherichia coli. However, Cm is needed for stable maintenance of pMC524/MBM in different hosts. In this study, the nucleotide sequences of these two plasmids were compared after sequencing of pMC524/MBM [EMBL Accession No. AJ312056 SAU312056]. Although these two plasmids have striking nucleotide sequence homology, the Plus Origin, Minus Origin, the replication protein (Rep), and the chloramphenicol acetyl transferase (Cat) have considerable variations. Possibly, these changes have modulated pMC524/MBM into an efficient shuttle-plasmid.

GENBANK AJ312056

EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)

Date Created: 20030507 Date Completed: 20030829 Latest Revision: 20061115

20221213

10.1007/s00284-002-3841-3

12732947