Characterization of the nodulation plasmid encoded chemoreceptor gene mcpG from Rhizobium leguminosarum.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Chemoreceptor Cells*; Bacterial Proteins/*genetics ; Membrane Proteins/*genetics ; Rhizobium leguminosarum/*genetics; Amino Acid Sequence ; Bacterial Proteins/metabolism ; Cloning, Molecular ; Membrane Proteins/metabolism ; Methyl-Accepting Chemotaxis Proteins ; Molecular Sequence Data ; Mutagenesis, Insertional ; Plasmids/genetics ; Sequence Homology, Amino Acid

Background: In general, chemotaxis in Rhizobium has not been well characterized. Methyl accepting chemotaxis proteins are sensory proteins important in chemotaxis of numerous bacteria, but their involvement in Rhizobium chemotaxis is unclear and merits further investigation.
Results: A putative methyl accepting chemotaxis protein gene (mcpG) of Rhizobium leguminosarum VF39SM was isolated and characterized. The gene was found to reside on the nodulation plasmid, pRleVF39d. The predicted mcpG ORF displayed motifs common to known methyl-accepting chemotaxis proteins, such as two transmembrane domains and high homology to the conserved methylation and signaling domains of well-characterized MCPs. Phenotypic analysis of mcpG mutants using swarm plates did not identify ligands for this putative receptor. Additionally, gene knockouts of mcpG did not affect a mutant strain's ability to compete for nodulation with the wild type. Notably, mcpG was found to be plasmid-encoded in all strains of R. leguminosarum and R. etli examined, though it was found on the nodulation plasmid only in a minority of strains.
Conclusions: Based on sequence homology R. leguminosarum mcpG gene codes for a methyl accepting chemotaxis protein. The gene is plasmid localized in numerous Rhizobium spp. Although localized to the sym plasmid of VF39SM mcpG does not appear to participate in early nodulation events. A ligand for McpG remains to be found. Apparent McpG orthologs appear in a diverse range of proteobacteria. Identification and characterization of mcpG adds to the family of mcp genes already identified in this organism.

Science. 1977 Apr 22;196(4288):434-6. (PMID: 17776952)
Appl Environ Microbiol. 1992 Apr;58(4):1153-8. (PMID: 16348685)
J Gen Microbiol. 1974 Sep;84(1):188-98. (PMID: 4612098)
Nature. 1983 Feb 17-23;301(5901):623-6. (PMID: 6402709)
Gene. 1993 May 15;127(1):15-21. (PMID: 8486283)
Appl Environ Microbiol. 1999 Jul;65(7):2833-40. (PMID: 10388672)
World J Microbiol Biotechnol. 1996 Mar;12(2):119-25. (PMID: 24415159)
Nature. 1997 May 22;387(6631):394-401. (PMID: 9163424)
Annu Rev Microbiol. 1985;39:89-108. (PMID: 3904617)
J Mol Biol. 2001 Jan 19;305(3):567-80. (PMID: 11152613)
J Bacteriol. 1995 Oct;177(20):5853-9. (PMID: 7592334)
Gene. 1993 Nov 30;134(1):89-91. (PMID: 8244036)
J Bacteriol. 1981 Nov;148(2):728-908 p. (PMID: 7298580)
Comput Appl Biosci. 1994 Dec;10(6):685-6. (PMID: 7704669)
J Bacteriol. 1981 Oct;148(1):193-202. (PMID: 7287623)
Gene. 1987;52(2-3):147-54. (PMID: 3038679)
Plant Physiol. 1988 Apr;86(4):1228-35. (PMID: 16666059)
J Bacteriol. 1985 Aug;163(2):417-22. (PMID: 4019407)
J Biol Chem. 1991 May 25;266(15):9746-53. (PMID: 2033064)
Science. 2001 Dec 14;294(5550):2317-23. (PMID: 11743193)
J Bacteriol. 1998 Mar;180(5):1009-22. (PMID: 9495737)
Adv Microb Physiol. 2001;45:157-98. (PMID: 11450109)
FEMS Microbiol Lett. 1999 Jul 1;176(1):111-6. (PMID: 10418137)
Microbiology (Reading). 1998 Jul;144 ( Pt 7):1945-1956. (PMID: 9695927)
Science. 2001 Jul 27;293(5530):668-72. (PMID: 11474104)
J Bacteriol. 1991 Sep;173(18):5837-42. (PMID: 1909323)
Plasmid. 1978 Sep;1(4):584-8. (PMID: 107540)
Can J Microbiol. 2001 Jun;47(6):495-502. (PMID: 11467725)
Gene. 1989 May 15;78(1):111-20. (PMID: 2548927)
Microbiol Rev. 1995 Mar;59(1):124-42. (PMID: 7708010)
Nature. 1999 Aug 19;400(6746):787-92. (PMID: 10466731)
Plasmid. 2000 Jul;44(1):34-43. (PMID: 10873525)
Adv Microb Physiol. 1999;41:229-89. (PMID: 10500847)
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. (PMID: 9254694)
Plant Physiol. 1992 Mar;98(3):1181-9. (PMID: 16668744)
J Bacteriol. 2002 Oct;184(19):5436-48. (PMID: 12218032)
J Bacteriol. 1992 Aug;174(16):5183-9. (PMID: 1644746)
J Bacteriol. 1989 Nov;171(11):6161-8. (PMID: 2553672)
Microbiology (Reading). 1994 Oct;140 ( Pt 10):2797-809. (PMID: 8000544)
J Bacteriol. 2000 Nov;182(21):6042-8. (PMID: 11029423)
Appl Environ Microbiol. 1984 Jul;48(1):149-52. (PMID: 6476827)
J Bacteriol. 2001 Aug;183(16):4681-6. (PMID: 11466269)
Mol Microbiol. 1990 Apr;4(4):567-74. (PMID: 2161988)
Mol Gen Genet. 1997 Oct;256(4):387-96. (PMID: 9393436)
Appl Environ Microbiol. 1992 Jul;58(7):2308-14. (PMID: 16348739)
Annu Rev Cell Dev Biol. 1997;13:457-512. (PMID: 9442881)
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. (PMID: 7984417)
Biochim Biophys Acta. 1996 Jul 31;1308(1):7-11. (PMID: 8765742)
Plasmid. 1985 Mar;13(2):99-105. (PMID: 4001194)

GENBANK AF141674

0 (Bacterial Proteins)
0 (Membrane Proteins)
0 (Methyl-Accepting Chemotaxis Proteins)

Date Created: 20030130 Date Completed: 20030430 Latest Revision: 20241121

20250114

PMC149452

10.1186/1471-2180-3-1

12553885