Anatomy of the planarian Dugesia japonica I. The muscular system revealed by antisera against myosin heavy chains.

Publisher: Zoological Society of Japan Country of Publication: Japan NLM ID: 8702287 Publication Model: Print Cited Medium: Print ISSN: 0289-0003 (Print) Linking ISSN: 02890003 NLM ISO Abbreviation: Zoolog Sci Subsets: MEDLINE

Original Publication: Tokyo, Japan : Zoological Society of Japan, c1984-

Immune Sera/*immunology ; Myosin Heavy Chains/*analysis ; Myosin Heavy Chains/*immunology ; Planarians/*anatomy & histology ; Planarians/*immunology; Animals ; Antibody Specificity ; Eye/anatomy & histology ; Eye/immunology ; Head/anatomy & histology ; Immunohistochemistry ; Intestines/anatomy & histology ; Intestines/immunology ; Mouth/anatomy & histology ; Mouth/immunology ; Muscles/anatomy & histology ; Muscles/immunology ; Organ Specificity ; Pharynx/anatomy & histology ; Pharynx/immunology ; Staining and Labeling

The planarian Dugesia japonica has two genes encoding myosin heavy chain, DjMHC-A and B (Kobayashi et al., 1998). We produced antibodies specifically recognizing each myosin heavy chain protein using their carboxyl terminal regions expressed in E. coli as antigens. Immunohistochemical analyses of sections and whole-mount specimens revealed the detailed structure and distribution of each type of muscle fiber in the planarian. In general, the MHC-A muscle fibers were distributed beneath the epithelial layers, namely, they were observable in the pharynx, the mouth, the intestine, the eyes and the body wall. In the pharynx, only MHC-A muscle fibers were present. In contrast, the MHC-B muscle fibers were distributed in the mesenchyme as dorso-ventral and transverse muscles, and in the body wall. The body-wall muscles were composed of an outer layer of circular MHC-A muscles and inner longitudinal and intermediate diagonal MHC-B muscle layers. Thus, two types of muscle fibers were distinguished by their distribution in the planarian.

0 (Immune Sera)
EC 3.6.4.1 (Myosin Heavy Chains)

Date Created: 20021112 Date Completed: 20030411 Latest Revision: 20131121

20221213

10.2108/zsj.19.1123

12426474