Evaluation of anti-oxidative, antimicrobial and anti-diabetic potential of Adiantum venustum and identification of its phytochemicals through GC-MS.

The present study aimed to explore antioxidant, antimicrobial and anti-diabetic properties of the fern Adiantum venustum. The TPC (total phenolic content) of methanolic extract of the plant was 247.95±0.0007μg of Gallic acid equivalents per gram of dried extract (mg GAE/g). The highest TPC was in n-butanolic fraction, which was 981.45±0.1562mg GAE/g. Hexane fraction showed lowest TPC (256.95±0.0420mg GAE/g). Ethyl acetate fraction exhibited highest total flavonoid content (TFC), i.e., 62.0±0.050mg of Rutin equivalents per gram of sample. DPPH (1,1- diphenyl-2- picryl hydrazyl) radical scavenging activity of the plant was significant. n-Butanolic fraction was most potent with IC50 being 1.06mg/mL. The IC50 of methanolic extract was 1.50mg/mL, that of aqueous fraction was 2.51 mg/mL, and that of chloroform fraction was 2.65mg/mL. Antibacterial potential of the fern was tested against two Grampositive bacterial strains, Pseudomonas aeruginosa and Escherichia coli and two Gram-negative bacterial strains, Bacillus subtilis and Staphylococcus aureus. n-Butanolic fraction showed highest zone of inhibition (ZOI, 25.13±1.237 mm) against P. aeruginosa. Ethyl acetate fraction was most active (ZOI, 15.75±1.060 mm) against S. aureus. Against E. coli, aqueous and n-butanolic fractions were most active (ZOI, 14.75±0.353 and 14.50±0.707mm, respectively). Minimum inhibitory concentration (MIC) of methanolic extract against B. subtilis was as low as 1.98mg/mL. The hexane fraction was least toxic against all the fractions. The plant displayed significant alpha-amylase inhibitory activity. Chloroform fraction was most effective with lowest IC50 (1.10mg/mL) followed by ethyl acetate fraction (1.92mg/mL). A. venustum is rich in phenolics and has substantial antioxidant, antimicrobial and alpha-amylase inhibitory potential. [ABSTRACT FROM AUTHOR]

Copyright of Pakistan Journal of Pharmaceutical Sciences is the property of Pakistan Journal of Pharmaceutical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)