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Membranes of retinal pigment epithelial cells in vitro are damaged in the phagocytotic process of the photoreceptor outer segment discs peroxidized by ferrous ions.

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  • معلومة اضافية
    • المصدر:
      Publisher: Munksgaard International Publishers Country of Publication: Denmark NLM ID: 8800247 Publication Model: Print Cited Medium: Print ISSN: 0893-5785 (Print) Linking ISSN: 08935785 NLM ISO Abbreviation: Pigment Cell Res Subsets: MEDLINE
    • بيانات النشر:
      Publication: Copenhagen : Munksgaard International Publishers
      Original Publication: New York : Alan R. Liss, Inc., c1987-
    • الموضوع:
      Hemoglobins/*toxicity ; Iron/*toxicity ; Oxidative Stress/*physiology ; Photoreceptor Cells/*drug effects ; Pigment Epithelium of Eye/*drug effects ; Retinal Degeneration/*etiology ; Retinal Hemorrhage/*complications; Animals ; Cattle ; Cell Membrane/drug effects ; Cell Membrane/pathology ; Cell Membrane/ultrastructure ; Cells, Cultured ; Microscopy, Confocal ; Microscopy, Electron ; Organ Culture Techniques ; Phagocytosis/drug effects ; Phagocytosis/physiology ; Photoreceptor Cells/pathology ; Photoreceptor Cells/ultrastructure ; Pigment Epithelium of Eye/pathology ; Pigment Epithelium of Eye/ultrastructure ; Retinal Degeneration/pathology ; Retinal Degeneration/physiopathology ; Swine
    • نبذة مختصرة :
      The ferrous ions released from haemoglobin and storage-transferrin ions cause oxidative stress in the eyes. We observed the phagocytotic process of the photoreceptor outer segment discs peroxidized by ferrous ions in the retinal pigment epithelial (RPE) cells in vitro, and investigated how the ferrous ions influenced RPE in vitro and the photoreceptor outer segment discs. We obtained isolated photoreceptor outer segment discs using sucrose gradient of specific gravity after homogenizing porcine retinas. After bovine RPE cells were cultured with isolated photoreceptor outer segment discs containing FeCl2 for 5 and 24 h, we incubated the specimens with rhodamine phalloidin, antimouse alpha-tubulin antibody and antimouse Ig G (FITC and rhodamine labelled). We observed the specimens by a laser scanning microscopy, and made the ultrathin sections with or without 2% uranyl acetate and 2% lead acetate for examination by transmission electron microscopy. Actin filaments and microtubules of specialized cells such as RPE cells were actively involved in phagocytosis of the photoreceptor outer segment discs. Microtubules were damaged during the phagocytotic process of the photoreceptor outer segment discs peroxidized by ferrous ions. The peroxidation increased the granular and aggregated autofluorescence of the photoreceptor outer segment discs. The membranes of the disc and the phagosomes, and lysosomes in RPE cells were damaged by ferrous ions and had fine particles with high electron density staining without uranium acetate and lead citrate. The cytoskeletons such as actin filaments and microtubules, and the membranes of the phagosomes and the lysosomes in RPE cells in vitro were damaged during the phagocytotic process of the photoreceptor outer segment discs peroxidized by ferrous ions.
    • الرقم المعرف:
      0 (Hemoglobins)
      E1UOL152H7 (Iron)
    • الموضوع:
      Date Created: 20020906 Date Completed: 20030212 Latest Revision: 20191025
    • الموضوع:
      20240829
    • الرقم المعرف:
      10.1034/j.1600-0749.2002.02054.x
    • الرقم المعرف:
      12213090