Molecular cloning and functional expression of bovine brain GABA transaminase.

Publisher: Korean Society for Molecular and Cellular Biology Country of Publication: United States NLM ID: 9610936 Publication Model: Print Cited Medium: Print ISSN: 1016-8478 (Print) Linking ISSN: 10168478 NLM ISO Abbreviation: Mol Cells Subsets: MEDLINE

Publication: <2014->: Seoul : Korean Society for Molecular and Cellular Biology
Original Publication: Seoul : Korean Society for Molecular Biology,

4-Aminobutyrate Transaminase/*metabolism ; Brain/*enzymology; 4-Aminobutyrate Transaminase/chemistry ; 4-Aminobutyrate Transaminase/genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Southern ; Brain/physiology ; Cattle ; Cloning, Molecular ; Humans ; Molecular Sequence Data ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/metabolism ; Sequence Alignment ; Tissue Distribution

We isolated a cDNA that encodes the bovine brain gamma-aminobutyrate transaminase (GABA-T; EC 2.6.1.19) from the lambda gt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial- expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

PIR AF305692

0 (Recombinant Fusion Proteins)
EC 2.6.1.19 (4-Aminobutyrate Transaminase)

Date Created: 20010920 Date Completed: 20020426 Latest Revision: 20240315

20240315

11561735