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Hemoglobin adducts of epoxybutene in workers occupationally exposed to 1,3-butadiene.

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  • المؤلفون: Begemann P;Begemann P; Srám RJ; Neumann HG
  • المصدر:
    Archives of toxicology [Arch Toxicol] 2001 Jan; Vol. 74 (11), pp. 680-7.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417615 Publication Model: Print Cited Medium: Print ISSN: 0340-5761 (Print) Linking ISSN: 03405761 NLM ISO Abbreviation: Arch Toxicol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Berlin, New York, Springer-Verlag.
    • الموضوع:
      Occupational Exposure*; Air Pollutants, Occupational/*adverse effects ; Butadienes/*adverse effects ; Carcinogens/*adverse effects ; Epoxy Compounds/*metabolism ; Hemoglobins/*drug effects; Butadienes/administration & dosage ; Carcinogens/administration & dosage ; Environmental Monitoring/methods ; Gas Chromatography-Mass Spectrometry ; Hemoglobins/metabolism ; Humans ; Inhalation Exposure ; Smoking/blood
    • نبذة مختصرة :
      1,3-Butadiene (BD) is an important chemical widely used in the synthetic rubber industry. Hemoglobin adducts of two of its reactive metabolites have been already investigated as possible parameters for exposure assessment. In this study hemoglobin adducts of epoxybutene (EB) were analyzed in blood samples from 17 workers in a BD monomer production unit and 19 controls in a heat production unit of a petrochemical plant near Prague, Czech Republic. BD exposure was determined by personal air sampling. The median level of exposure was 440 microg/m3 (range < 11-17 mg/m3) for the exposed workers and < 6 microg/m3 (< 5-150 microg/m3) for the controls. The adduct N-(2-hydroxy-3-butenyl)valine (HBVal) formed by the reaction of the N-terminal valine of globin with carbon-1 of EB was measured. The N-alkylated amino acid was analyzed by gas chromatography/mass spectrometry (GC/MS) after degradation by the modified Edman procedure. Using published methods problems arose with high background levels, especially in the negative ion chemical ionization (NCI) mode. In the present study a limit of detection of 0.2 pmol/g globin was achieved by using 400 mg globin, a variation in extraction solvents, an additional purification step and a widely extended GC temperature program. The median hemoglobin adduct level of the Czech BD monomer production workers (0.7 pmol/g globin; n = 17) was significantly higher than that of the controls (0.2 pmol/g globin; n = 19; P<0.05). Smoking controls showed higher hemoglobin adduct levels than nonsmoking controls (P<0.1) and significantly higher BD exposure levels (P<0.01).
    • الرقم المعرف:
      0 (Air Pollutants, Occupational)
      0 (Butadienes)
      0 (Carcinogens)
      0 (Epoxy Compounds)
      0 (Hemoglobins)
      478ERR5NKR (3,4-epoxy-1-butene)
      JSD5FGP5VD (1,3-butadiene)
    • الموضوع:
      Date Created: 20010224 Date Completed: 20010412 Latest Revision: 20190906
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1007/s002040000183
    • الرقم المعرف:
      11218044