Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles.

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المؤلفون: Yunxia Luan^1,2 ; Jiayi Chen^1,2 ; Cheng Li^1,2 ; Gang Xie³ ; Hailong Fu^1,2 ; Zhihong Ma^1,2 ; Anxiang Lu^1,2
المصدر:
Toxins. Dec2015, Vol. 7 Issue 12, p5377-5385. 9p.
الموضوع:
*COLORIMETRIC analysis; *OCHRATOXINS; *APTAMERS; *GOLD nanoparticles; *POLYMERS

معلومة اضافية
- نبذة مختصرة :
  A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection. [ABSTRACT FROM AUTHOR]