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5'-Flanking sequence and promoter activity of the rabbit neuronal nitric oxide synthase (nNOS) gene.

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اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Jeong Y;Jeong Y; Won J; Kim C; Yim J
  • المصدر:
    Molecules and cells [Mol Cells] 2000 Oct 31; Vol. 10 (5), pp. 566-74.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Korean Society for Molecular and Cellular Biology Country of Publication: United States NLM ID: 9610936 Publication Model: Print Cited Medium: Print ISSN: 1016-8478 (Print) Linking ISSN: 10168478 NLM ISO Abbreviation: Mol Cells Subsets: MEDLINE
    • بيانات النشر:
      Publication: <2014->: Seoul : Korean Society for Molecular and Cellular Biology
      Original Publication: Seoul : Korean Society for Molecular Biology,
    • الموضوع:
      Promoter Regions, Genetic*; 5' Untranslated Regions/*genetics ; Brain/*enzymology ; Nitric Oxide Synthase/*genetics; Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Codon/genetics ; DNA, Complementary ; DNA-Binding Proteins/metabolism ; Molecular Sequence Data ; Nerve Tissue Proteins/genetics ; Nitric Oxide Synthase Type I ; Rabbits ; Transcription, Genetic
    • نبذة مختصرة :
      We have isolated a rabbit neuronal nitric oxide synthase (nNOS) cDNA encoding a protein of 1,435 amino acids. Using the cDNA clones as probes, the 5'-flanking region of the nNOS gene was isolated from a rabbit genomic DNA library. 5'RACE and primer extension analysis of rabbit brain total RNA mapped multiple transcription initiation sites localized 474-487 bp upstream from the translation start codon. Analysis of 5,197 bp of the 5'-flanking sequence revealed that the rabbit nNOS gene promoter lacks canonical TATA or CCAAT boxes and, instead, contains a GC-rich region and multiple Sp1 sites. Farther from the +1start, various putative cis-elements including AP-1, AP-4, NF-kappaB, STAT, CREB, C/EBP and c-Myc were observed. The functional promoter activity of the 5'-flanking region was demonstrated by its ability to drive the expression of a beta-galactosidase reporter gene in several cell types. Serial deletion analysis of the promoter region revealed that the -291 to -172 region, which contains two Sp1 sites, is essential for basal transcriptional activity. These results suggest that the rabbit nNOS promoter contains characteristics of inducible genes.
    • الرقم المعرف:
      0 (5' Untranslated Regions)
      0 (Codon)
      0 (DNA, Complementary)
      0 (DNA-Binding Proteins)
      0 (Nerve Tissue Proteins)
      EC 1.14.13.39 (Nitric Oxide Synthase)
      EC 1.14.13.39 (Nitric Oxide Synthase Type I)
    • الموضوع:
      Date Created: 20001202 Date Completed: 20010531 Latest Revision: 20240315
    • الموضوع:
      20240315
    • الرقم المعرف:
      10.1007/s10059-000-0566-7
    • الرقم المعرف:
      11101149