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XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.

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  • المؤلفون: Maiorano D;Maiorano D; Moreau J; Méchali M
  • المصدر:
    Nature [Nature] 2000 Apr 06; Vol. 404 (6778), pp. 622-5.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print Cited Medium: Print ISSN: 0028-0836 (Print) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
    • بيانات النشر:
      Publication: Basingstoke : Nature Publishing Group
      Original Publication: London, Macmillan Journals ltd.
    • الموضوع:
      Saccharomyces cerevisiae Proteins*; Cell Cycle Proteins/*physiology ; DNA Replication/*physiology ; DNA-Binding Proteins/*physiology; Animals ; Cell Cycle Proteins/metabolism ; Cell Nucleus/physiology ; Chromatin/physiology ; Cloning, Molecular ; Minichromosome Maintenance Complex Component 4 ; Molecular Sequence Data ; Nuclear Proteins ; Protein Binding ; Replication Origin/physiology ; Schizosaccharomyces/physiology ; Xenopus Proteins ; Xenopus laevis
    • نبذة مختصرة :
      In eukaryotic cells, chromosomal DNA replication begins with the formation of pre-replication complexes at replication origins. Formation and maintenance of pre-replication complexes is dependent upon CDC6 (ref. 1), a protein which allows assembly of MCM2-7 proteins, which are putative replicative helicases. The functional assembly of MCM proteins into chromatin corresponds to replication licensing. Removal of these proteins from chromatin in S phase is crucial in origins firing regulation. We have identified a protein that is required for the assembly of pre-replication complexes, in a screen for maternally expressed genes in Xenopus. This factor (XCDT1) is a relative of fission yeast cdt1, a protein proposed to function in DNA replication, and is the first to be identified in vertebrates. Here we show, using Xenopus in vitro systems, that XCDT1 is required for chromosomal DNA replication. XCDT1 associates with pre-replicative chromatin in a manner dependent on ORC protein and is removed from chromatin at the time of initiation of DNA synthesis. Immunodepletion and reconstitution experiments show that XCDT1 is required to load MCM2-7 proteins onto pre-replicative chromatin. These findings indicate that XCDT1 is an essential component of the system that regulates origins firing during S phase.
    • Comments:
      Comment in: Nature. 2000 Apr 6;404(6778):560-1. (PMID: 10766226)
    • Molecular Sequence:
      GENBANK AJ250122
    • الرقم المعرف:
      0 (Cdt1 protein, Xenopus)
      0 (Cell Cycle Proteins)
      0 (Chromatin)
      0 (DNA-Binding Proteins)
      0 (Nuclear Proteins)
      0 (Saccharomyces cerevisiae Proteins)
      0 (Xenopus Proteins)
      EC 3.6.4.12 (MCM4 protein, S cerevisiae)
      EC 3.6.4.12 (MCM4 protein, human)
      EC 3.6.4.12 (Minichromosome Maintenance Complex Component 4)
    • الموضوع:
      Date Created: 20000415 Date Completed: 20000427 Latest Revision: 20131121
    • الموضوع:
      20221213
    • الرقم المعرف:
      10.1038/35007104
    • الرقم المعرف:
      10766247