A novel isolated Terrabacter-like bacterium can mineralize 2-aminopyrimidine, the principal metabolite of microbial sulfadiazine degradation.

Recently we showed that during the degradation of sulfadiazine (SDZ) by Microbacterium lacus strain SDZm4 the principal metabolite 2-aminopyrimidine (2-AP) accumulated to the same molar amount in the culture as SDZ disappeared (Tappe et al. Appl Environ Microbiol 79:2572-2577, ). Although 2-AP is considered a recalcitrant agent, long-term lysimeter experiments with C-pyrimidine labeled SDZ ([C]SDZ) provided indications for substantial degradation of the pyrimidine moiety of the SDZ molecule. Therefore, we aimed to enrich 2-AP degrading bacteria and isolated a pure culture of a Terrabacter-like bacterium, denoted strain 2APm3. When provided with C-labeled SDZ, M. lacus strain SDZm4 degraded [C]SDZ to [C]2-AP. Resting cells of 2APm3 at a concentration of 5 × 10 cells ml degraded 62 µM [C]2-AP to below the detection limit (0.6 µM) within 5 days. Disappearance of 2-AP resulted in the production of at least two transformation products (M1 and M2) with M2 being identified as 2-amino-4-hydroxypyrimidine. After 36 days, the transformation products disappeared and 83 % of the applied [C]2-AP radioactivity was trapped as CO. From this we conclude that a consortium of two species should be able to almost completely degrade SDZ in soils. [ABSTRACT FROM AUTHOR]

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