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Bulk RNA degradation by nitrogen starvation-induced autophagy in yeast.
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- المؤلفون: Huang, Hanghang1; Kawamata, Tomoko2; Horie, Tetsuro2; Tsugawa, Hiroshi1; Nakayama, Yasumune1; Ohsumi, Yoshinori2; Fukusaki, Eiichiro1
- المصدر:
EMBO Journal. Jan2015, Vol. 34 Issue 2, p154-168. 15p. 1 Color Photograph, 1 Diagram, 1 Chart, 6 Graphs.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Autophagy is a catabolic process conserved among eukaryotes. Under nutrient starvation, a portion of the cytoplasm is non-selectively sequestered into autophagosomes. Consequently, ribosomes are delivered to the vacuole/lysosome for destruction, but the precise mechanism of autophagic RNA degradation and its physiological implications for cellular metabolism remain unknown. We characterized autophagy-dependent RNA catabolism using a combination of metabolome and molecular biological analyses in yeast. RNA delivered to the vacuole was processed by Rny1, a T2-type ribonuclease, generating 3′- NMPs that were immediately converted to nucleosides by the vacuolar non-specific phosphatase Pho8. In the cytoplasm, these nucleosides were broken down by the nucleosidases Pnp1 and Urh1. Most of the resultant bases were not re-assimilated, but excreted from the cell. Bulk non-selective autophagy causes drastic perturbation of metabolism, which must be minimized to maintain intracellular homeostasis. [ABSTRACT FROM AUTHOR]
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