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METHODS FOR DETECTING ESTRONE BY MASS SPECTROMETRY

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اقرأ أكثر حفظ في قائمتي
  • Publication Date:
    January 16, 2020
  • معلومة اضافية
    • Document Number:
      20200018772
    • Appl. No:
      16/579438
    • Application Filed:
      September 23, 2019
    • نبذة مختصرة :
      Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.
    • Claim:
      1. A method for determining the amount of estrone in a sample, said method comprising: (a) adding deuterated estrone to a sample; (b) derivatizing estrone to produce a derivative of estrone; (c) purifying the sample by high performance liquid chromatography (HPLC); (d) ionizing the derivative of estrone and deuterated estrone in said sample to produce one or more ions detectable by mass spectrometry; and (e) detecting the amount of the ions by tandem mass spectrometry.
    • Claim:
      2. The method of claim 1, wherein said deuterated estrone is d4-estrone.
    • Claim:
      3. The method of claim 1, wherein said deuterated estrone is 2,4,16,16-d4 estrone.
    • Claim:
      4. The method of claim 1, wherein said method further comprises subjecting estrone from said sample to an extraction.
    • Claim:
      5. The method of claim 1, wherein said method has a limit of quantitation less than or equal to 20 pg/mL.
    • Claim:
      6. The method of claim 1, wherein said method has a limit of detection less than or equal to 10 pg/mL.
    • Claim:
      7. The method of claim 1, wherein said sample is a human plasma or serum sample.
    • Claim:
      8. The method of claim 1, wherein said ionization is atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI).
    • Claim:
      9. A method for determining the amount of estrone in a sample, said method comprising: (a) adding deuterated estrone to a sample; (b) derivatizing estrone to produce a derivative of estrone; (c) purifying the sample by liquid chromatography; (d) ionizing derivatized estrone and deuterated estrone in said sample to produce one or more ions detectable by mass spectrometry; and (e) detecting the amount of the ions by tandem mass spectrometry.
    • Claim:
      10. The method of claim 9, wherein said liquid chromatography comprises high turbulence liquid chromatography (HTLC).
    • Claim:
      11. The method of claim 9, wherein said liquid chromatography comprises high performance liquid chromatography (HPLC).
    • Claim:
      12. The method of claim 9, wherein said deuterated estrone is d4-estrone.
    • Claim:
      13. The method of claim 9, wherein said deuterated estrone is 2,4,16,16-d4 estrone.
    • Claim:
      14. The method of claim 9, wherein said method further comprises subjecting estrone from said sample to an extraction.
    • Claim:
      15. The method of claim 9, wherein said method has a limit of quantitation less than or equal to 20 pg/mL.
    • Claim:
      16. The method of claim 9, wherein said method has a limit of detection less than or equal to 10 pg/mL.
    • Claim:
      17. The method of claim 9, wherein said sample is a human plasma or serum sample.
    • Claim:
      18. The method of claim 9, wherein said ionization is atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI).
    • Current International Class:
      01; 01
    • الرقم المعرف:
      edspap.20200018772