Trajectory analysis reveals the differentiation pathway of swine trophoblasts.

A) UMAPs showing Slingshot calculated trajectory of trophoblasts within sTO. Top panel shows the trajectory across labeled clusters, whereas bottom panel shows the trajectory across a pseudotime. B) Heatmap showing temporally regulated genes across the calculated pseudotime trajectory. Individual columns represent individual cells that are ordered along the calculated pseudotime. Identities are positions along the pseudotime are shown above the heatmap. C) Representative expression plots showing gene expression changes along the calculated pseudotime that are enriched in the proliferative stem cell population. D) Representative expression plots and spatial feature plots showing genes that are enriched during the transition to Interface-Trophoblasts and their expression pattern within our spatial transcriptomics dataset. E, F) Representative expression plots and spatial feature plots showing genes that are enriched during the transition to Areola-2 trophoblasts and their expression pattern within our spatial transcriptomics dataset. The underlying data for this figure can be found at the Gene Expression Omnibus via accession numbers GSM8980980, GSM8980981, GSM8980982, GSM8980983, GSM8980992, GSM8980993, GSM8980994.