Tissue-specific analysis of INSL3 mRNA via qPCR assays in adult hamster and effects of sex-steroid hormones during estrous cycle.

(A) A panel of tissues was collected and examined in this study, including the lung, liver, duodenum, cerebellum, testis, ovary, adrenals, HG, hypothalamus, spleen, epididymis, uterus, and pancreas. (B) Relative expression profiles were determined during different stages of the estrous cycle such as proestrus P, estrus E, metestrus M, and diestrus D. The INSL3 gene under study was normalized using ACTB as the housekeeping gene. The means with the asterisks are statistically significantly different between males (in blue) and females (in pink). The statistical significance was calculated using a one-way analysis of variance (ANOVA), followed by Sidak´s and Tukey’s test ( p < 0.05). Data are shown as means ± SD (n = 5). **** indicate p < 0.0001, *** indicate p < 0.001.