Screenıng for anti-quorum sensing and anti-biofilm activity in viscum album l. extracts and its biochemical composition

Many opportunistic pathogenic bacteria use the Quorum Sensing (QS) system to coordinate their virulenceexpressions. Thus, QS can likely be a new approach to control bacterial infections. The aim of this study was to evaluate theantimicrobial, anti-biofilm, and anti-quorum sensing activities of ethanol, chloroform, and dichloromethane: methanol extractsof leaf, stem, and fruits of the European mistletoe Viscum album L. on 2 Gram-positive and 7 Gram-negative pathogenicbacteria. The extracts at concentrations ranging from 50 to 250mg/ml were tested depending on the extracts of the plant partsand the test bacteria. The extract with 50mg/ml concentration, in which no antimicrobial activity was observed, was used foranti-quorum sensing and antibiofilm studies. The dichloromethane: methanol extracts were found to show the highest biologicalactivities. QS activities of the plant extracts were also determined using the recently established Chromobacterium violaceumCV026 reporter strain and the signaling molecule N-(ß-ketocaproyl)-L-homoserine lactone (3-oxo-C6-HSL) agar welldiffusion assay. Biofilm was quantified using the microtiter plate test and the crystal violet assay. Anti-microbial, anti-biofilm,and anti-quorum sensing activity of leaf and stem extracts showed higher efficiency than fruit extracts. It was concluded thatthe extracts of V. album had the potential to treat microbial infections by biofilm inhibition or inhibition of QS. ; Fırsatçı patojenik bakteriler, virülans ifadelerini koordine etmek için Quorum Sensing (QS) sistemini kullanır. Dolayısıyla QS sistemi, bakteriyel enfeksiyonların kontrolü için yeni bir yaklaşım olarak tercih edilebilir. Bu çalışmanın amacı, 2 Gram-pozitif, 7 Gram-negatif patojenik bakteri üzerinde analiz edilen Viscum album L. bitkisinin gövde, yaprak ve meyve gibi bölümlerine ait etanol, kloroform ve diklorometan:metanol ekstraktlarının antimikrobiyal, anti-biyofilm ve anti-quorum sensing aktivitelerinin değerlendirilmesidir. Kullanılan bitki parçası ekstraktına ve test ...