Additional file 1 of Multi-modal imaging of high-risk ductal carcinoma in situ of the breast using C2Am: a targeted cell death imaging agent

Additional file 1: Figure S1 Fluorescence imaging (a) of C2Am and iC2Am standard solutions. Excitation (dashed) and emission (filled) profiles (b) of iC2Am-650 (Ex/Em: 652|672 nm, red, left) and C2Am-750 (Ex/Em: 753|783 nm, brown, right). Serial dilutions (decreasing concentration, left to right) of the imaging probes (c, e) were imaged on an IVIS200™ system and the mean fluorescence intensity (in radiant efficiency) correlated with probe concentration, in the far red (d) and near infra-red NIR (f), for C2Am (open circles) and iC2Am (closed squares). iC2Am fluorescence (d) in the far-red (black squares) was minimal (ca. 10% of that of C2Am, open circles). C2Am fluorescence (f) in the NIR was undetectable. Fluorescence intensities (g) of C2Am-650 (in the far-red, open circles in g, data in 2nd row of c) and iC2Am-750 (in the NIR, closed squares in g, data in the top row of e) were identical at all concentrations. A 1:1 molar mixture (h) of C2Am|iC2Am (c, e, lower row) was also imaged in the far-red (open circles) and NIR (closed squares) showing equivalent readouts at all concentrations, in the range 40-2,500 nM. Figure S2. Single wavelength OA images of (a) phantoms (b) and in vivo tumor models are illustrated with the regions of interest (ROIs) used for the data analysis outlined. (c) OA spectra of iC2Am and C2Am molecules were obtained from multispectral imaging of the probes using tissue mimicking phantoms.