METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation.

Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE

Publication: 2005- : Abingdon, Oxford : Taylor & Francis
Original Publication: New York, N.Y. : Marcel Dekker, c1990-

Adipocytes*/cytology ; Adipocytes*/physiology ; Adipocytes*/metabolism ; Cell Differentiation*/genetics ; Cell Differentiation*/physiology ; Methyltransferases*/genetics ; Methyltransferases*/metabolism ; Adipogenesis*/genetics ; Adipogenesis*/physiology; Signal Transduction/genetics ; Animals ; Cattle ; Gene Knockdown Techniques ; Gene Expression Regulation

m6A methylation is the most common mRNA modification in mammals and plays a significant role in regulating various biological functions. Some studies have demonstrated that the methyltransferase METTL3 can promote adipogenesis. However, the regulatory mechanisms of METTL14 and WTAP, both methyltransferases, in adipogenesis remain unclear. This study investigated their effects on bovine preadipocyte differentiation using siRNA-mediated knockdown combined with transcriptomic analysis. Silencing METTL14 and WTAP significantly impaired lipid droplet formation and revealed distinct regulatory pathways: METTL14 knockdown affected genes like JAK2 and STAT3, while WTAP suppression down-regulated PPARγ/FABP4 signalling pathway components. These findings demonstrate that WTAP specifically modulates bovine adipocyte differentiation through the PPARγ/FABP4 pathway.

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Keywords: Bovine; Cell differentiation; METTL14; Preadipocytes; WTAP

EC 2.1.1.- (Methyltransferases)

Date Created: 20250317 Date Completed: 20250513 Latest Revision: 20251211

20260130

PMC12674308

10.1080/10495398.2025.2476531

40094566