A single gene in Fusarium oxysporum limits host range.

Publisher: Blackwell Science in collaboration with the British Society of Plant Pathology Country of Publication: England NLM ID: 100954969 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1364-3703 (Electronic) Linking ISSN: 13643703 NLM ISO Abbreviation: Mol Plant Pathol Subsets: MEDLINE

Original Publication: Oxford, UK : Blackwell Science in collaboration with the British Society of Plant Pathology, c2000-

Citrullus/*microbiology ; Cucumis sativus/*microbiology ; Cucurbitaceae/*microbiology ; Fungal Proteins/*metabolism ; Fusarium/*genetics ; Host Specificity/*genetics ; Plant Diseases/*microbiology; Citrullus/immunology ; Cucumis sativus/immunology ; Cucurbitaceae/immunology ; Fungal Proteins/genetics ; Fusarium/pathogenicity ; Plant Diseases/immunology ; Plant Immunity

Fusarium oxysoporum f. sp. radicis-cucumerinum (Forc) is able to cause disease in cucumber, melon, and watermelon, while F. oxysporum f. sp. melonis (Fom) can only infect melon plants. Earlier research showed that mobile chromosomes in Forc and Fom determine the difference in host range between Forc and Fom. By closely comparing these pathogenicity chromosomes combined with RNA-sequencing data, we selected 11 candidate genes that we tested for involvement in the difference in host range between Forc and Fom. One of these candidates is a putative effector gene on the Fom pathogenicity chromosome that has nonidentical homologs on the Forc pathogenicity chromosome. Four independent Forc transformants with this gene from Fom showed strongly reduced or no pathogenicity towards cucumber, while retaining pathogenicity towards melon and watermelon. This suggests that the protein encoded by this gene is recognized by an immune receptor in cucumber plants. This is the first time that a single gene has been demonstrated to determine a difference in host specificity between formae speciales of F. oxysporum.
(© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)

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Keywords: Fusarium oxysoporum f. sp. radicis-cucumerinum; Fusarium oxysporum f. sp. melonis; cucurbits; effector-triggered immunity (ETI); host range

0 (Fungal Proteins)

Fusarium oxysporum

Date Created: 20201104 Date Completed: 20210712 Latest Revision: 20210712

20260130

PMC7749751

10.1111/mpp.13011

33146465