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Functional activities of the C. d. ruruima venom pools: Venom samples from Cdr-SVMP, Cdr-CTX, C. d. terrificus, and B. jararaca were subjected to the hemorrhagic activity test by measuring the halo induced in the dorsal skin of mice three hours after the intradermal injection of 50 μg of the venoms (A); myotoxic activity by measuring creatine kinase levels in the serum of mice three hours after the injection of 20 μg of the venoms into the gastrocnemius muscle (B); and procoagulant activity, expressed as the increase in absorbance at 650 nm due to clot formation over five minutes, as indicated in the Methods section (C).
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