Silencing Exosomal circ102927 Inhibits Foot Melanoma Metastasis via Regulating Invasiveness, Epithelial-Mesenchymal Transition and Apoptosis

Huiying Wan,1 Ling Zhong,1 Tian Xia,2 Dingding Zhang3 1Department of Dermatology, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 2Department of Pathology, Air Force Hospital of Western Theater Command, Chengdu, People’s Republic of China; 3Sichuan Provincial Key Laboratory for Genetic Disease, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, People’s Republic of ChinaCorrespondence: Tian Xia, Department of Pathology, Air Force Hospital of Western Theater Command, No. 1 Gongnongyuan Street, Jinjiang District, Chengdu City, Sichuan Province, 610031, People’s Republic of China, Tel +86-18030761839, Email yours_summer2023@126.com Dingding Zhang, Sichuan Provincial Key Laboratory for Genetic Disease, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, No. 32 West Second Section of First Ring Road, Qingyang District, Chengdu City, Sichuan Province, 610072, People’s Republic of China, Tel +86-18981838863, Email zhangdd25@126.comBackground: Exosomes contain abundant circular RNAs (circRNAs), playing an important role in intercellular communication. However, the function and underlying molecular mechanism of exosomal circRNAs in foot metastatic melanoma remain unclear.Methods: Twelve differentially expressed exosomal circRNAs between patients with metastatic and primary foot melanoma were screened through high-throughput sequencing, and their expression levels were detected by the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). CircRNA102927 silencing and overexpression A2058 cell line was constructed, and the effects of circRNA102927 on cell proliferation, apoptosis, migration, invasion, and epithelial-mesenchymal transition (EMT) were assessed using cell counting kit-8 (CCK-8), flow ...