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Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges.

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • الموضوع:
      2020
    • Collection:
      Sistema Sanitario Público de Andalucía (SSPA): Repositorio
    • نبذة مختصرة :
      Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10 μL of serum or plasma without any extraction steps, with a limit of detection of 1.32 pM that enabled the identification of DILI. Testing of 192 human serum samples showed that DCL accurately identified patients at risk of DILI after acetaminophen overdose (area under ROC curve 0.98 (95% CI; 0.96-1), P
    • ISSN:
      1520-6882
    • Relation:
      http://hdl.handle.net/10668/14958; http://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81
    • الرقم المعرف:
      10.1021/acs.analchem.9b05449
    • الدخول الالكتروني :
      https://doi.org/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81
      https://doi.org/10.1021/acs.analchem.9b05449
      http://hdl.handle.net/10668/14958
      https://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81
    • Rights:
      open access
    • الرقم المعرف:
      edsbas.3F700E9D