Low-level laser activates Wnt/β-catenin signaling pathway-promoting hair follicle stem cell regeneration and wound healing: Upregulate the expression of key downstream gene Lef 1.

Publisher: Blackwell Country of Publication: England NLM ID: 9504453 Publication Model: Print Cited Medium: Internet ISSN: 1600-0846 (Electronic) Linking ISSN: 0909752X NLM ISO Abbreviation: Skin Res Technol Subsets: MEDLINE

Publication: <1998-> : Oxford : Blackwell
Original Publication: Copenhagen, Denmark ; Cambridge, MA : Munksgaard, c1995-

Hair Follicle*/radiation effects ; Wound Healing*/radiation effects ; Wound Healing*/physiology ; Wnt Signaling Pathway*/physiology ; Wnt Signaling Pathway*/radiation effects ; Stem Cells*/radiation effects ; Stem Cells*/metabolism ; Lymphoid Enhancer-Binding Factor 1*/metabolism ; Lymphoid Enhancer-Binding Factor 1*/genetics ; Up-Regulation* ; Cell Proliferation*/radiation effects ; Low-Level Light Therapy*/methods ; Regeneration*/physiology ; Regeneration*/radiation effects; Animals ; Mice ; beta Catenin/metabolism ; Humans

Background: The objective of this study is to investigate the mechanism by which low-level laser stimulation promotes the proliferation of intraepithelial hair follicle stem cells (HFSCs) in wounds. This research aims to expand the applications of laser treatment, enhance wound repair methods, and establish a theoretical and experimental foundation for achieving accelerated wound healing.
Methods: The experimental approach involved irradiating a cell model with low-level laser to assess the proliferation of HFSCs and examine alterations in the expression of proteins related to the Wnt/β-catenin signaling pathway. A mouse back wound model was established to investigate the effects of low-level laser irradiation on wound healing rate, wound microenvironment, and the proliferation of HFSCs in relation to the Wnt/β-catenin signaling pathway.
Results: The research findings indicate that low-level laser light effectively activates the Wnt signaling pathway, leading to the increased accumulation of core protein β-catenin and the upregulation of key downstream gene Lef 1. Consequently, this regulatory mechanism facilitates various downstream biological effects, including the notable promotion of HFSC proliferation and differentiation into skin appendages and epithelial tissues. As a result, the process of wound healing is significantly accelerated.
Conclusion: Low levels of laser activates the Wnt signalling pathway, promotes the regeneration of hair follicle stem cells and accelerates wound healing.
(© 2024 The Author(s). Skin Research and Technology published by John Wiley & Sons Ltd.)

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2023byfy008 Natural Science Projects at Bengbu Medical University; 2022AH051451 Natural Science Research Project of Anhui Educational Committee; KJ2021A0772 Natural Science Research Project of Anhui Educational Committee; KJ2019A0345 Natural Science Research Project of Anhui Educational Committee; Central Laboratory of The First Affiliated Hospital of Bengbu Medical University

Keywords: Wnt/β‐catenin pathway; carcinogenesis; low‐level laser; stem cells; wound healing

0 (Lymphoid Enhancer-Binding Factor 1)
0 (beta Catenin)
0 (Lef1 protein, mouse)

Date Created: 20240618 Date Completed: 20240618 Latest Revision: 20240620

20240620

PMC11182782

10.1111/srt.13807

38887112