Accelerated full-thickness skin wound tissue regeneration by self-crosslinked chitosan hydrogel films reinforced by oxidized CNC-AgNPs stabilized Pickering emulsion for quercetin delivery.

Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE

Original Publication: London : BioMed Central, 2003-

Quercetin*/chemistry ; Quercetin*/pharmacology ; Wound Healing*/drug effects ; Chitosan*/chemistry ; Emulsions*/chemistry ; Skin*/drug effects ; Skin*/injuries ; Metal Nanoparticles*/chemistry ; Silver*/chemistry ; Hydrogels*/chemistry; Animals ; Mice ; Humans ; Biocompatible Materials/chemistry ; Bandages ; Drug Liberation ; Drug Delivery Systems/methods ; Cellulose/chemistry ; Male ; Regeneration/drug effects ; HaCaT Cells ; Oxidation-Reduction ; Methylgalactosides

Background: The non-toxic self-crosslinked hydrogel films designed from biocompatible materials allow for controlled drug release and have gathered remarkable attention from healthcare professionals as wound dressing materials. Thus, in the current study the chitosan (CS) film is infused with oil-in-water Pickering emulsion (PE) loaded with bioactive compound quercetin (Qu) and stabilized by dialdehyde cellulose nanocrystal-silver nanoparticles (DCNC-AgNPs). The DCNC-AgNPs play a dual role in stabilizing PE and are involved in the self-crosslinking with CS films. Also, this film could combine the advantage of the controlled release and synergistic wound-healing effect of Qu and AgNPs.
Results: The DCNC-AgNPs were synthesized using sodium periodate oxidation of CNC. The DCNC-AgNPs were used to stabilize oil-in-water PE loaded with Qu in its oil phase by high speed homogenization. Stable PEs were prepared by 20% v/v oil: water ratio with maximum encapsulation of Qu in the oil phase. The Qu-loaded PE was then added to CS solution (50% v/v) to prepare self-crosslinked films (CS-PE-Qu). After grafting CS films with PE, the surface and cross-sectional SEM images show an inter-penetrated network within the matrix between DCNC and CS due to the formation of a Schiff base bond between the reactive aldehyde groups of DCNC-AgNPs and amino groups of CS. Further, the addition of glycerol influenced the extensibility, swelling ratio, and drug release of the films. The fabricated CS-PE-Qu films were analyzed for their wound healing and tissue regeneration potential using cell scratch assay and full-thickness excisional skin wound model in mice. The as-fabricated CS-PE-Qu films showed great biocompatibility, increased HaCat cell migration, and promoted collagen synthesis in HDFa cells. In addition, the CS-PE-Qu films exhibited non-hemolysis and improved wound closure rate in mice compared to CS, CS-Qu, and CS-blank PE. The H&E staining of the wounded skin tissue indicated the wounded tissue regeneration in CS-PE-Qu films treated mice.
Conclusion: Results obtained here confirm the wound healing benefits of CS-PE-Qu films and project them as promising biocompatible material and well suited for full-thickness wound healing in clinical applications.
(© 2024. The Author(s).)

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2022R1I1A1A01065269 National Research Foundation of Korea; 2018R1A6A1A03025582 National Research Foundation of Korea

Keywords: Chitosan hydrogel film; Dialdehyde cellulose nanocrystal; Pickering emulsion; Quercetin; Silver nanoparticles; Wound healing

9IKM0I5T1E (Quercetin)
9012-76-4 (Chitosan)
0 (Emulsions)
3M4G523W1G (Silver)
0 (Hydrogels)
0 (Biocompatible Materials)
0 (hydrogel film)
9004-34-6 (Cellulose)
0 (Methylgalactosides)

Date Created: 20240607 Date Completed: 20240608 Latest Revision: 20240610

20240610

PMC11162036

10.1186/s12951-024-02596-0

38849931