Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
Improving dermal fibroblast-to-epidermis communications and aging wound repair through extracellular vesicle-mediated delivery of Gstm2 mRNA.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : BioMed Central, 2003-
- الموضوع:
- نبذة مختصرة :
Skin aging is characterized by the disruption of skin homeostasis and impaired skin injury repair. Treatment of aging skin has long been limited by the unclear intervention targets and delivery techniques. Engineering extracellular vesicles (EVs) as an upgraded version of natural EVs holds great potential in regenerative medicine. In this study, we found that the expression of the critical antioxidant and detoxification gene Gstm2 was significantly reduced in aging skin. Thus, we constructed the skin primary fibroblasts-derived EVs encapsulating Gstm2 mRNA (EVs Gstm2 ), and found that EVs Gstm2 could significantly improve skin homeostasis and accelerate wound healing in aged mice. Mechanistically, we found that EVs Gstm2 alleviated oxidative stress damage of aging dermal fibroblasts by modulating mitochondrial oxidative phosphorylation, and promoted dermal fibroblasts to regulate skin epidermal cell function by paracrine secretion of Nascent Polypeptide-Associated Complex Alpha subunit (NACA). Furthermore, we confirmed that NACA is a novel skin epidermal cell protective molecule that regulates skin epidermal cell turnover through the ROS-ERK-ETS-Cyclin D pathway. Our findings demonstrate the feasibility and efficacy of EVs-mediated delivery of Gstm2 for aged skin treatment and unveil novel roles of GSTM2 and NACA for improving aging skin.
(© 2024. The Author(s).)
- References:
Aging Dis. 2013 Dec 05;5(4):263-73. (PMID: 25110610)
Nat Med. 2014 Aug;20(8):847-56. (PMID: 25100530)
J Allergy Clin Immunol. 2004 Aug;114(2):422-8. (PMID: 15316527)
Oncogene. 2003 Oct 20;22(47):7369-75. (PMID: 14576844)
J Nanobiotechnology. 2020 Aug 14;18(1):113. (PMID: 32799868)
Cell Metab. 2020 Jul 7;32(1):71-86.e5. (PMID: 32574561)
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7293-7. (PMID: 3174634)
Sci Rep. 2014 Dec 18;4:7535. (PMID: 25520167)
Biomed Pharmacother. 2022 Jul;151:113102. (PMID: 35594716)
Mol Cell Biol. 1998 Mar;18(3):1312-21. (PMID: 9488446)
J Biol Chem. 2020 Aug 21;295(34):12203-12213. (PMID: 32641493)
J Invest Dermatol. 2014 Jun;134(6):1570-1578. (PMID: 24441101)
Geroscience. 2022 Jun;44(3):1871-1878. (PMID: 35399134)
Pharmaceutics. 2020 Sep 03;12(9):. (PMID: 32899309)
J Cell Sci. 2005 Apr 15;118(Pt 8):1595-605. (PMID: 15784678)
Antioxidants (Basel). 2021 Jan 08;10(1):. (PMID: 33430157)
Biogerontology. 2022 Dec;23(6):681-698. (PMID: 35727468)
Int J Mol Sci. 2017 Sep 25;18(10):. (PMID: 28946661)
Eur J Pharm Biopharm. 2016 Aug;105:69-74. (PMID: 27257030)
Aging (Albany NY). 2020 Nov 17;12(22):22599-22613. (PMID: 33201836)
Nucleic Acids Res. 2022 Jan 7;50(D1):D27-D38. (PMID: 34718731)
ACS Nano. 2020 Sep 22;14(9):12133-12147. (PMID: 32790341)
Biochem Biophys Rep. 2022 Jan 27;29:101213. (PMID: 35128081)
J Biol Chem. 2000 Dec 1;275(48):37838-45. (PMID: 10982809)
Evid Based Complement Alternat Med. 2020 Jan 13;2020:3121936. (PMID: 32015752)
Immunology. 2004 Jul;112(3):397-403. (PMID: 15196207)
Acta Biomater. 2016 Jan;30:246-257. (PMID: 26602823)
Mol Cell. 2019 Sep 5;75(5):996-1006.e8. (PMID: 31377116)
Ann Dermatol. 2022 Jun;34(3):163-172. (PMID: 35721334)
Autophagy. 2014 Apr;10(4):618-30. (PMID: 24434817)
Science. 2023 Jun 23;380(6651):1238-1243. (PMID: 37347872)
Methods Mol Biol. 2021;2318:241-254. (PMID: 34019294)
Int J Nanomedicine. 2020 Oct 19;15:7979-7993. (PMID: 33116513)
Tissue Eng Part B Rev. 2012 Oct;18(5):341-62. (PMID: 22462759)
Stem Cells. 2004;22(5):812-22. (PMID: 15342945)
Stem Cell Res Ther. 2021 Jan 22;12(1):77. (PMID: 33482923)
ACS Nano. 2019 Oct 22;13(10):11273-11282. (PMID: 31449388)
Int J Pharm Investig. 2015 Oct-Dec;5(4):192-9. (PMID: 26682189)
J Hepatol. 2022 Feb;76(2):407-419. (PMID: 34656650)
Drug Metab Rev. 2011 May;43(2):236-52. (PMID: 21323602)
Front Oncol. 2018 Jun 13;8:221. (PMID: 29951374)
Adv Drug Deliv Rev. 2020;159:332-343. (PMID: 32305351)
Stem Cell Res Ther. 2018 Jun 13;9(1):159. (PMID: 29895333)
Pharmaceuticals (Basel). 2020 Oct 01;13(10):. (PMID: 33019732)
Am J Pathol. 2001 Oct;159(4):1255-62. (PMID: 11583953)
Am J Respir Crit Care Med. 2009 Apr 1;179(7):601-7. (PMID: 19151192)
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1091-6. (PMID: 9448290)
Genomics Proteomics Bioinformatics. 2021 Aug;19(4):578-583. (PMID: 34400360)
Gut. 2009 Jan;58(1):5-15. (PMID: 18664505)
Biology (Basel). 2019 May 11;8(2):. (PMID: 31083540)
Nat Commun. 2019 Jun 3;10(1):2387. (PMID: 31160572)
Stem Cell Res Ther. 2021 Mar 11;12(1):172. (PMID: 33706803)
Arthritis Res Ther. 2018 Jun 7;20(1):115. (PMID: 29880011)
Stem Cell Res Ther. 2014 Jan 30;5(1):19. (PMID: 24480247)
Aging Cell. 2012 Aug;11(4):668-74. (PMID: 22577861)
Oncotarget. 2017 Dec 24;9(5):5588-5599. (PMID: 29464020)
J Control Release. 2023 Apr;356:402-415. (PMID: 36858264)
Arch Dermatol Res. 2015 May;307(4):351-64. (PMID: 25740152)
Nature. 1994 Aug 11;370(6489):434-40. (PMID: 8047162)
Biochem Biophys Res Commun. 2017 Nov 18;493(2):1102-1108. (PMID: 28919421)
Biomed Microdevices. 2016 Aug;18(4):67. (PMID: 27422107)
Adv Healthc Mater. 2020 Jun;9(11):e2000198. (PMID: 32338465)
J Biol Chem. 2004 Oct 15;279(42):43893-9. (PMID: 15299025)
Cell Stem Cell. 2016 Dec 1;19(6):725-737. (PMID: 27641305)
- Grant Information:
82070365 the National Natural Science Foundation of China; JCYJ20190807103407873 the Basic Research General Program of Shenzhen
- Contributed Indexing:
Keywords: Extracellular vesicles (EVs); Glutathione S-transferase mu 2 (GSTM2); Nascent polypeptide-associated complex alpha subunit (NACA); Needle free injection; Wound healing
- الرقم المعرف:
EC 2.5.1.18 (Glutathione Transferase)
0 (RNA, Messenger)
EC 2.5.1.18 (glutathione S-transferase Mu 2)
- الموضوع:
Date Created: 20240602 Date Completed: 20240602 Latest Revision: 20240605
- الموضوع:
20240605
- الرقم المعرف:
PMC11145791
- الرقم المعرف:
10.1186/s12951-024-02541-1
- الرقم المعرف:
38825668
No Comments.