Twist2 contributes to skin regeneration and hair follicle formation in mouse fetuses.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Hair Follicle*/metabolism ; Regeneration*/genetics ; Skin*/metabolism ; Twist-Related Protein 2*/metabolism ; Twist-Related Protein 2*/genetics ; Wound Healing*/genetics ; Wound Healing*/physiology; Animals ; Mice ; Fetus/metabolism ; Fibroblasts/metabolism ; Mice, Inbred ICR ; Mice, Knockout ; Repressor Proteins ; Twist-Related Protein 1

Unlike adult mammalian wounds, early embryonic mouse skin wounds completely regenerate and heal without scars. Analysis of the underlying molecular mechanism will provide insights into scarless wound healing. Twist2 is an important regulator of hair follicle formation and biological patterning; however, it is unclear whether it plays a role in skin or skin appendage regeneration. Here, we aimed to elucidate Twist2 expression and its role in fetal wound healing. ICR mouse fetuses were surgically wounded on embryonic day 13 (E13), E15, and E17, and Twist2 expression in tissue samples from these fetuses was evaluated via in situ hybridization, immunohistochemistry, and reverse transcription-quantitative polymerase chain reaction. Twist2 expression was upregulated in the dermis of E13 wound margins but downregulated in E15 and E17 wounds. Twist2 knockdown on E13 left visible marks at the wound site, inhibited regeneration, and resulted in defective follicle formation. Twist2-knockdown dermal fibroblasts lacked the ability to undifferentiate. Furthermore, Twist2 hetero knockout mice (Twist + /-) formed visible scars, even on E13, when all skin structures should regenerate. Thus, Twist2 expression correlated with skin texture formation and hair follicle defects in late mouse embryos. These findings may help develop a therapeutic strategy to reduce scarring and promote hair follicle regeneration.
(© 2024. The Author(s).)

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JP 20K18422 Japan Society for the Promotion of Science

Keywords: Hair follicle formation; Scar; Skin regeneration; Twist2; Wound healing

0 (Repressor Proteins)
0 (Twist-Related Protein 1)
0 (Twist-Related Protein 2)
0 (Twist2 protein, mouse)

Date Created: 20240513 Date Completed: 20240513 Latest Revision: 20240711

20240711

PMC11091223

10.1038/s41598-024-60684-5

38740788