Adipose stem cell-derived exosomes promote wound healing by regulating the let-7i-5p/GAS7 axis.

Publisher: Blackwell Science Country of Publication: England NLM ID: 101130964 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-2165 (Electronic) Linking ISSN: 14732130 NLM ISO Abbreviation: J Cosmet Dermatol Subsets: MEDLINE

Original Publication: Oxford, UK : Blackwell Science, c2002-

Adipose Tissue*/cytology ; Cell Movement*/drug effects ; Exosomes*/metabolism ; Hydrogen Peroxide*/pharmacology ; MicroRNAs*/metabolism ; MicroRNAs*/genetics ; Wound Healing*/drug effects; Animals ; Humans ; Rats ; Cell Survival/drug effects ; Disease Models, Animal ; HaCaT Cells ; Keratinocytes/physiology ; Keratinocytes/metabolism ; Rats, Sprague-Dawley ; Stem Cells/metabolism ; Stem Cells/physiology

Background: Injury to skin tissue is devastating for human health, making it imperative to devise strategies for hastening wound healing. Normal wound healing is a complex process comprising overlapping steps, including hemostasis, inflammatory response, proliferation, and matrix remodeling. This study investigated the effects of adipose stem cell-derived exosomes (ADSC-exos) on wound healing and the underlying mechanisms.
Methods: In vitro hydrogen peroxide (H 2 O 2 )-treated human keratinocyte (HaCaT) cell lines and in vivo animal wound models were established for this purpose. The cell migration was assessed using transwell and wound healing assays, while exosome biomarker expressions were studied using western blot. Moreover, adipose stem cells were identified using flow cytometry, alizarin red S and oil red O staining, and transmission electron microscopy.
Results: Results indicated that H 2 O 2 treatment inhibited the cell viability and migration of HaCaT cells while being promoted by ADSC-exos. Mechanistic investigations revealed that microRNA-let-7i-5p (let-7i-5p) in ADSC-exos was carried into the HaCaT cells, inhibiting the expression of growth arrest-specific-7 (GAS7). Rescue experiments further verified these results, which indicated that GAS7 overexpression reversed the effect of let-7i-5p on the viability and migration of HaCaT cells, suggesting ADSC-exos promoted wound healing via the let-7i-5p/GAS7 axis.
Conclusion: Adipose stem cell-derived-exos enhanced the viability and migration of HaCaT via carrying let-7i-5p and targeting GAS7, ultimately promoting wound healing in rats.
(© 2024 The Authors. Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.)

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Keywords: GAS7; adipose stem cell; exosome; let‐7i‐5p; wound healing

BBX060AN9V (Hydrogen Peroxide)
0 (MicroRNAs)
0 (mirnlet7 microRNA, human)
0 (Gas7 protein, rat)
0 (GAS7 protein, human)
0 (MIRNLET7 microRNA, rat)

Date Created: 20240302 Date Completed: 20240518 Latest Revision: 20240524

20240524

10.1111/jocd.16267

38429909