Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Cell Proliferation/*radiation effects ; Cesium Radioisotopes/*toxicity ; DNA Breaks, Double-Stranded/*radiation effects ; X-Ray Therapy/*adverse effects ; X-Rays/*adverse effects; DNA Damage/radiation effects ; DNA Repair/genetics ; Humans ; Lung/pathology ; Lung/radiation effects

To clarify the health risks of internal radiation exposure, it is important to investigate the radiological effects of local exposure at cell levels from radioactive materials taken up by organs. Focusing on the response of cell populations post-irradiation, X-ray microbeams are very effective at reproducing the effects of local exposure within an internal exposure in vitro. The present study aims to clarify the effects of local exposure by investigating the response of normal human cell (MRC-5) populations irradiated with X-ray microbeams of different beam sizes to DNA damage. The populations of MRC-5 were locally irradiated with X-ray microbeams of 1 Gy at 0.02-1.89 mm ² field sizes, and analyzed whether the number of 53BP1 foci as DSB (DNA double strand break) per cell changed with the field size. We found that even at the same dose, the number of DSB per cell increased depending on the X-irradiated field size on the cell population. This result indicated that DNA damage repair of X-irradiated cells might be enhanced in small size fields surrounded by non-irradiated cells. This study suggests that X-irradiated cells received some signal (a rescue signal) from surrounding non-irradiated cells may be involved in the response of cell populations post-irradiation.

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0 (Cesium Radioisotopes)
4T2E65IAR7 (Cesium-137)

Date Created: 20210327 Date Completed: 20211020 Latest Revision: 20231111

20231215

PMC7997867

10.1038/s41598-021-86416-7

33772061