Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
N2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
- المؤلفون: Wang, Can1 (AUTHOR); Ulryck, Nathalie1 (AUTHOR); Herzel, Lydia2 (AUTHOR); Pythoud, Nicolas3 (AUTHOR); Kleiber, Nicole2 (AUTHOR); Guérineau, Vincent4 (AUTHOR); Jactel, Vincent5 (AUTHOR); Moritz, Chloé3 (AUTHOR); Bohnsack, Markus T2,6 (AUTHOR); Carapito, Christine3 (AUTHOR); Touboul, David4,7 (AUTHOR); Bohnsack, Katherine E2 (AUTHOR) ; Graille, Marc1 (AUTHOR)
- المصدر:
Nucleic Acids Research. 8/11/2023, Vol. 51 Issue 14, p7496-7519. 24p.
- الموضوع:
- معلومة اضافية
- نبذة مختصرة :
Modified nucleotides in non-coding RNAs, such as tRNAs and snRNAs, represent an important layer of gene expression regulation through their ability to fine-tune mRNA maturation and translation. Dysregulation of such modifications and the enzymes installing them have been linked to various human pathologies including neurodevelopmental disorders and cancers. Several methyltransferases (MTases) are regulated allosterically by human TRMT112 (Trm112 in Saccharomyces cerevisiae), but the interactome of this regulator and targets of its interacting MTases remain incompletely characterized. Here, we have investigated the interaction network of human TRMT112 in intact cells and identify three poorly characterized putative MTases (TRMT11, THUMPD3 and THUMPD2) as direct partners. We demonstrate that these three proteins are active N 2-methylguanosine (m2G) MTases and that TRMT11 and THUMPD3 methylate positions 10 and 6 of tRNAs, respectively. For THUMPD2, we discovered that it directly associates with the U6 snRNA, a core component of the catalytic spliceosome, and is required for the formation of m2G, the last 'orphan' modification in U6 snRNA. Furthermore, our data reveal the combined importance of TRMT11 and THUMPD3 for optimal protein synthesis and cell proliferation as well as a role for THUMPD2 in fine-tuning pre-mRNA splicing. [ABSTRACT FROM AUTHOR]
No Comments.